Emily Wiley Associate Professor of Biology   Email: ewiley@jsd.claremont.edu Office: Keck Science Center 210 Phone: 909-607-9698 Office Hours: on Sabbatical 2008-2009 Web Site: http://sites.google.com/site/wileylabs/about-dr-wiley     Courses Taught This Year: Fall BIOL 043L Introductory Biology Section 1   MWF 9:00-9:50 <> BC 36       Fall BIOL 170L Molecular Biology Section 1   TTh 12:00-1:10 <> KS 125       Spring BIOL 170L Molecular Biology Section 1   TTh 12:00-1:10 <> KS 125       Educational Background: B.A., Western Washington University Ph.D., University of Washington Postdoc, University of Rochester; Fred Hutchinson Cancer Research Center Courses Taught: Molecular Biology (Bio170L) Molecular Seminar (Bio173L) Biochemistry (Bio177) Introductory Biology (cell/molecular/genetics/physiology) and Introductory Laboratory (Bio43L) Research Interests: My lab studies the relationships between histone modifications, chromatin structure and the control of gene expression using the protozoan Tetrahymena thermophila as a model system. Most of our work is concentrated on investigating the role of histone deacetylases in chromatin dynamics and heterochromatin assembly. Thesis Topics: Cellular localization of histone deacetylase enzymes through GFP-tagging; Engineering gene knockout constructs of chromatin modification enzymes, generation of knockout cell lines, and phenotype characterization of mutant cells; Identification of protein complexes that modify chromatin; Mechanistic roles for histone deacetylase enzymes in the control of gene expression and other cellular processes   Selected Publications List: Click to open new window. 1.   Sean R. Gallagher and Emily A. Wiley . (2008). Current Protocols: Essential Laboratory Techniques. (Gallagher, S.R. and Wiley, E.A, eds.) Wiley and Sons, Inc. : .   2.   Coyne, R.S., Thiagarajan, M., Jones, K.M., Wortman, J.R., Tallon, L.J., Haas, B.J., Cassidy-Hanley, D.M., Wiley, E.A., et al . (2008). Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure. BMC Genomics   9: 562-579.   3.   Smith, J., Torigoe, S., Maxson, J., Fish, L., and Wiley, E.A . (2008). A class II HDAC deacetylates newly-synthesized histones in Tetrahymena. Eukaryotic Cell   7(3): 471-482.   4.   Parker, K., Maxson, J., Mooney, A., and Wiley, E.A . (2007). Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila. Eukaryotic Cell   6: 1913-1924.   5.   Wiley, E.A., Myers, T., Parker, K., Braun, T., Yao, M.-C . (2005). The class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila. Eukaryotic Cell   4: 981-990. Abstract   6.   Wiley, E.A., Mizzen, C., Allis, C.D . (2000). Isolation and characterization of in vivo modified histones and an activity gel assay for identification of histone acetyltransferases. . Methods in Cell Biology: Tetrahymena thermophila. (James Forney and David Asai, eds.) Academic Press 62: 379-394.   7.   Wiley, E.A., Ohba, R., Yao, M.-C., Allis, C.D . (2000). Developmentally regulated Rpd3p homolog specific to the transcriptionally active macronucleus of vegetative Tetrahymena thermophila. Molecular and Cellular Biology   23: 8319-8328. Abstract   8.   Huang, H., Smothers, J.F., Wiley, E.A., Allis, C.D . (1999). A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila. Molecular and Cellular Biology   19: 3624-3634. Abstract   9.   Huang, H., Wiley, E.A., Lending, C.R., Allis, C.D . (1998). An HP1-like protein is missing from transcriptionally silent micronuclei of Tetrahymena.. Proceedings of the National Academy of Science   95: 13624-13629. Abstract